Avtor/Urednik     Bajrović, Fajko
Naslov     Dolgoročni učinki neugodnih razmer ob poškodbi perifernega živca na uspeh regeneracije
Tip     monografija
Kraj izdaje     Ljubljana
Založnik     Univerza v Ljubljani, Medicinska fakulteta
Leto izdaje     1992
Obseg     str. 45
Jezik     slo
Abstrakt     Early axonal regeneration after nerve crush proceeds fairly well even in the absence of Schwann cells distally to the site of lesion. The aim of the present investigation was to examine possible longterm detrimental effects of elimination of the Schwann cell support at the time ofnerve lesion on motor and sensory axon regeneration. In one experimental group, freezing-thawing was applied to a 25 mm segment of the rat sciatic nerve distally to the crush to destroy the cells. Animals in the second group, in addition received Dexamethasone throughout the 8 day period after nerve crush and freezing in order to inhibit NGF production in the proximal nerve stump. Animals with axonotmesis served as controls. Recovery of toe-spread reflex of the hind paw was followed during the 4 month period after crush and, in parallel reappearance of pain response on the foot was tested by the pinch test. Finally, the number of axons proximally and distally to the lesion site was determined in nerve cross-sections using immunohistochemical reaction to neurofilaments. Pain response of the foot in both experimental groups reappeared 7 - 8 days later than in control animals, representing 30 - 40% delay. Thereafter, recovery of the responsivnes along the foot proceeded with the same rate and pattern in all groups of animals. Four months after nerve lesion, complete recovery of the pain response on the sole was observed in all animals whereas 3 - 7% of the dorsal foot surface, pertaining to the previosly cut n. saphenous, stil remained insensitiv to pain stimuli. Toe-spread reflex in the two experimental groups started to recover about a week later than in the control animals but recovery was complete thereafter and persisted throughout the observed period. (Abstract truncated at 2000 characters).
Deskriptorji     SCIATIC NERVE
NERVE REGENERATION
DEXAMETHASONE
AXONS
NERVE GROWTH FACTORS
RATS, WISTAR
SENSORY THRESHOLDS
IMMUNOHISTOCHEMISTRY