Avtor/Urednik		Žnidaršič, Nada
Naslov		Dinamika bakrovih in cinkovih zvrsti ter z njo povezani celični mehanizmi v prebavilu raka enakonožca Porcellio scaber (Crustacea: Isopoda)
Prevedeni naslov		Copper and zinc dynamics and related cellular mechanisms in the digestive system of isopod crustacean Porcellio scaber (Crustacea: Isopoda)
Tip		monografija
Kraj izdaje		Ljubljana
Založnik		Biotehniška fakulteta
Leto izdaje		2003
Obseg		str. 88
Jezik		slo
Abstrakt		Isopods accumulate metals in the digestive glands (hepatopancreas). Crustaceans display an enormously active copper metabolism, as copper is part of their respiratory pigment hemocyanin. Metallothioneins (MT) are involved in the homeostasis of essential metals. Intracellular granules are considered as cellular storage sites for metals. Histochemical and biochemical methods were used to investigate the distribution of intracellular copper granules in the hepatopancreas and to study the copperand zinc-binding MT-like proteins in the hepatopancreas and the hindgut of Porcedlio scaber. We have analysed animals in different phases of the moulting cycle and animals exposed to different concentrations of zinc in food. Our study revealed, that the amount of copper granules in the hepatopancreas varied among animals, but this was not related to moulting phases. The distribution of copper granules was similar in all four gland tubules. This indicates, that processes, related to the accumulation of copper in the granules, are alike in all tubules. The anterior parts of the hepatopancreatic tubules contained more copper granules than posterior parts. Our results support the assumption, that intracellular copper granules in the hepatopancreatic S cells contain copper-thiolate complexes, probably Cu-MT. Analyses of the animals from the stock culture indicate the presence of Cu-MT-like proteins in the cytosol and in the organelles of the hepatopancreatic cells. The amount of copper- and zinc-binding MT-like proteins in the cytosol of the hindgut cells was increased in animals exposed to zinc-dosed food, which points out the involvement of these proteins in the response of the animal to elevated contentration of zinc in food.
Deskriptorji		CRUSTACEA DIGESTIVE SYSTEM COPPER ZINC MOLTING FOOD CONTAMINATION METALLOTHIONEIN GLUTATHIONE HISTOCYTOCHEMISTRY CHROMATOGRAPHY, HIGH PRESSURE LIQUID MICROSCOPY, FLUORESCENCE