Avtor/Urednik		Pangršič, Tina; Stušek, Peter; Belušič, Gregor; Zupančič, Gregor
Naslov		Light dependence of oxygen consumption by blowfly eyes recorded with a magnetic diver balance
Tip		članek
Vir		J Comp Physiol (A)
Vol. in št.		Letnik 191
Leto izdaje		2005
Obseg		str. 75-84
Jezik		eng
Abstrakt		We measured the oxygen (O2) consumption of isolated blowfly eyes using a magnetic diver balance, a device for high-resolution volumetric O2 consumption measurements. The light-induced O2 consumption is at most three times the value of the dark consumption, which is 0.6 nl O2 s-1 eye-1, and is in good agreement with the estimates based on electrophysiological data. With longer stimuli the increase follows a double exponential time course. The respective time constants are approximately 2 and 20 s and show no dependence on light intensity, whereas the dependence of amplitudes can be fitted by a Hill equation. Decreasing the stimulus duration reveals that the peak in O2 consumption overshoots the time course induced by long stimuli. We suggest this may be a general feature of mitochondrial activation. The dependence of the O2 consumption peak on stimulus duration at high light intensity has a hump with stimulus durations of 10-20 ms, coinciding with the stimulus durations that start to induce the adaptation of the receptor potential.
Deskriptorji		PHOTORECEPTORS, INVERTEBRATE OXYGEN CONSUMPTION MITOCHONDRIA ELECTROPHYSIOLOGY DIPTERA