Avtor/Urednik		Arsov, Zoran; Schara, Milan; Zorko, Matjaž; Štrancar, Janez
Naslov		The membrane lateral domain approach in the studies of lipid-protein interaction of GPI-anchored bovine erythrocyte acetylcholinesterase
Tip		članek
Vir		Eur Biophys J
Vol. in št.		Letnik 33
Leto izdaje		2004
Obseg		str. 715-25
Jezik		eng
Abstrakt		A novel membrane lateral domain approach was used to test whether the activity of the membrane-bound enzyme acetylcholinesterase (AChE) depends on the local properties (e.g. local lipid ordering) of bovine erythrocyte-ghost membrane. This issue has an additional aspect of interest due to an alternative mode of insertion of AChE molecules into the membrane by the glycosylphosphatidylinositol (GPI) anchor. In our experiments the lateral domain membrane structure was influenced by temperature and by the addition of n-butanol, and was quantitatively characterized using the method of EPR spectrum decomposition. The activity of AChE was determined by a colorimetric assay in the same samples. The results show that the membrane stabilizes the conformation of the membrane-bound AChE compared to the isolated AChE. In addition, a correlation was observed between the temperature dependence of order parameter of the most-ordered domain type and the activity of AChE. Therefore, our findings support the idea that the function of GPI proteins can be modulated by the lipid bilayer. Based on the assumption that the overall activity of AChE depends on the order parameters of particular domain types as well as their proportions, two models for AChE activity were introduced. In the first, a random distribution of enzyme molecules was proposed, and in the second, localization of enzyme molecules in a single (cholesterol-rich) domain type was assumed. Better agreement between measured and calculated activity values speaks in favor of the second model.
Deskriptorji		ERYTHROCYTE MEMBRANE ACETYLCHOLINESTERASE GLYCOSYLPHOSPHATIDYLINOSITOLS ALCOHOLS, BUTYL ACETYLTHIOCHOLINE CATTLE TEMPERATURE DITHIONITROBENZOIC ACID LIPID BILAYERS