Avtor/Urednik		Vrtačnik-Bokal, E; Rakar, S; Možina, A; Poljak, M
Naslov		Human papillomavirus infection in relation to mild dyskaryosis in conventional cervical cytology
Tip		članek
Vir		Eur J Gynaecol Oncol
Vol. in št.		Letnik 26, št. 1
Leto izdaje		2005
Obseg		str. 39-42
Jezik		eng
Abstrakt		Purpose of investigation: To establish the prevalence and distribution of high-risk human papillomavirus (HPV) genotyps in Slovene women with repeat mild dyskaryosis, and to evaluate three molecular methods for the detection of HPV that could be used as a complementary method to cervical cytology. Methods: In this prospective study 148 women with three subsequent cervical cytologic tests within two years showing mild dyskaryosis were enrolled. HPV infection was determined using three molecular tests: Hybrid Capture Il and two variants of polymerase chain reaction (PCR-PGMY11/PGMY09 and PCR-CPI/CPIIG). Results: HPV was detected in 17 of the 45 women aged < 30 years and in 21 of the 103 women aged > 30 years (37.8% vs 20.4%, p = 0.04). The most common genotype was HPV 16 detected in eight (21.1%) women, the next were NPV 53 and HPV 51, each detected in five (13.2%) women. The three molecular methods matched in 92.9%. Conclusion: Low prevalence of HPV infections indicates that cervical screening programmes in Slovenia are overburdened with mild dyskaryosis. Repeat cytology is not reliable; HPV testing might be useful as a complementary method.
Deskriptorji		PAPILLOMAVIRUS, HUMAN CERVICAL INTRAEPITHELIAL NEOPLASIA VAGINAL SMEARS GENOTYPE POLYMERASE CHAIN REACTION PROSPECTIVE STUDIES