Avtor/Urednik     Štempelj, Mateja
Naslov     Mehanizem sproščanja histamina iz mastocitov podgane po aktivaciji z živčnim rastnim dejavnikom
Tip     monografija
Kraj izdaje     Ljubljana
Založnik     Univerza v Ljubljani, Medicinska fakulteta
Leto izdaje     2004
Obseg     str. 78
Jezik     slo
Abstrakt     Mast cells play a central role in the inflammatory and allergic reactions. In response to immunologic or non-immunologic stimuli they release histamin and a variety of inflammatory and immuno-regulatory mediators. Nerve growth factor (NGF) is essential for the survival of sensory and sympathetic neurones and has a wide range of other effects. It has been shown, that NGF induces degranulation of mast cells by interacting with its high-affinity receptor of a TrkA-type. In the first group of our experiments, we studied the effect of extracellular Na+ and Ca2+ ions on NGF induced histamine release from mast cells. We have found that the removal of extracellular Na+ ions reduces histamine release induced by NGF in the medium containing physiological (1 mM) concentration of Ca2+ ions. In contrast, in the medium containig low (0.1 mM) concentration of Ca2+ ions, the removal of Na+ ions from the medium enhances histamine release elicited by NGF. In the second group of experiments, we evaluated the effect of amiloride, an inhibitor of Na+/Ca2+ exchanger, and 5-N,N-hexamethylene amiloride, an inhibitor of Na+/H+ exchanger, on NGF induced histamine release. Our results indicate, that changing the concentration of extracellular Na+ and Ca2+ ions regulates the activity of Na+/Ca2+ and Na+/H+ exchangers in mast cell membrane. Na+/Ca2+ exchanger regulates the concentration of cytosolic calcium, whereas Na+/H+ exchanger plays an important role in intracellular pH regulation. Combined action of these exchange mechanisms serves to modify histamine release from mast cells. (Abstract truncated at 2000 characters)
Deskriptorji     MAST CELLS
HISTAMINE RELEASE
NERVE GROWTH FACTORS
HISTAMINE
CALCIUM
SODIUM
AMILORIDE
PROTEIN KINASE C
PROTEIN-TYROSINE KINASE
PHOSPHOLIPASE C
CALMODULIN-DEPENDENT PROTEIN KINASES
RATS, WISTAR