Avtor/Urednik		Vovko, Tomaž
Naslov		In vitro označevanje prionskih leh z 18-fluoro-2-dialkilamino-6-acilmalononitril naftalenom (FDDNP)
Tip		monografija
Kraj izdaje		Ljubljana
Založnik		Univerza v Ljubljani, Medicinska fakulteta
Leto izdaje		2004
Obseg		str. 33
Jezik		slo
Abstrakt		Background: Prion diseases or transmissible spongiform encephalopathies (TSE) are inevitably lethal neurodegenerative diseases, with a typical pattern of pathological prion protein deposits in patients' brains. The deposits often possess structural characteristics of amyloid and are termed prion plaques. Currently there is no reliable ante mortem diagnostic test for confirmation of TSE. A compound 2-(1-{6-[(2-fluoroethyl)(methyl)amino]-2-nafthyl}ethyliden) malononitrile (FDDNP ) is a hydrophobic, fluorescent molecule, whose radioactive analogue is currently being evaluated as a positron emission tomography (PET) probe for in vivo diagnosis of Alzheimer disease (AD). In tissue sections from AD cases, FDDNP labels senile plaques and neurofibrillary tangles, which possess amyloid characteristics. Aim: We aimed to determine potential applicability of FDDNP in the diagnostics of TSE and assess if prion plaque labeling correlates with their amyloid characteristics. Hypotheses: We posed two work hypotheses: 1. FDDNP would successfully label prion plaques in brain tissue sections of TSE patients, and 2. FDDNP deposit labeling would correlate with their amyloid characteristics. Methods: We compared FDDNP labeling of prion deposits with standard histochemical methods for amyloid labeling and with specific immunochemical methods for pathological prion protein detection in brain tissue sections of individuals who died of sporadic Creutzfeldt-Jakob disease (sCJD), Gerstmann Straussler Sheinker (GSS) disease and the new variant CJD (vCJD). Using a computerized image analysis system we compared labeling of the same deposits by different methods. We determined the fraction of all prion plaques in the section, which labeled with FDDNP. Using a Chi-square test and setting the significance threshold at p<0.001, we also determined the correlation between the amyloid characteristics of the deposits and their FDDNP labeling. (Abstract truncated at 2000 characters).
Deskriptorji		CREUTZFELDT-JAKOB SYNDROME GERSTMANN-STRAUSSLER SYNDROME PRIONS BRAIN IMMUNOCHEMISTRY IMAGE PROCESSING, COMPUTER-ASSISTED AUTOPSY NAPHTHALENES