Avtor/Urednik		Stibilj, Janina
Naslov		Uporaba metod molekularne biologije pri identifikaciji netuberkuloznih mikobakterij v humani medicini
Prevedeni naslov		Use of molekular methods for the identification of nontuberculous mycobacteria in human medicine
Tip		monografija
Kraj izdaje		Ljubljana
Založnik		Univerza v Ljubljani, Biotehniška fakulteta, Oddelek za biologijo
Leto izdaje		2005
Obseg		str. 69
Jezik		slo
Abstrakt		A continued growth in the number of isolated nontuberculous mycobacteria (NTM) can be observed worldwide. NTM can be a cause of disease in patients with either preexisting lung disease or deficient immune systems. Yearly between 200 and 250 NTM isolates are isolated from 150 patients in Slovenia. In the Laboratory for mycobacteria of Golnik Hospital, Slovenia, in only 5-10 cases per year the diagnosis of NTM disease is confirmed. Thus the exact identification of NTM is of clinical and therapeutic significance. In our study the efficiency of traditional methods, a hybridisation technique (AccuProbe test) and a nucleic acid amplification technique (GenoType Mycobacterium test) were compared. Based on traditional methods 21/50 isolates (42%) were identified to the species level, 11/50 more (22%) to the complex level, with a total of 64% of NTM isolates. Traditional identification is lengthy, experienced workers are required and it does not permit the separation amongst species of M. avium complex or the identification of M. peregrinum, M. celatum and M. malmoense. With AccuProbe test 16/50 isolates (32%) were identified to the species level. Short duration (2-3 h), relative simplicity and especially the possibility to distinguish amongst species of the M. avium complex are the main merits of the test. However it covers only a narrow range of the most common clinically significant NTM species, while xperimentally tested GenoType Mycobacterium test covers more mycobacterial species. From 38 (70%) with this test identified isolates, 35 were identified to the species level and 4 to the complex level. Only with GenoType Mycobacterium test it was possible to identify M. malmoense, M. peregrinum and M. celatuna. Long duration, possible false positive results and inability to distinguish among M. chelorzae and M. abscessus were the principal negative points of this test. Molecular methods are therefore an important tool for improvement of NTM identifications.
Deskriptorji		MYCOBACTERIUM MYCOBACTERIUM AVIUM MYCOBACTERIUM CHELONAE MYCOBACTERIUM AVIUM COMPLEX PHENOTYPE GENOTYPE MYCOBACTERIUM, ATYPICAL