Avtor/Urednik		Maružin, Mitja
Naslov		Vpliv proteaznih inhibitorjev naravnega izvora na invazivnost rakastih celičnih linij B16 in MCF10AT in vitro
Tip		monografija
Kraj izdaje		Ljubljana
Založnik		Univerza v Ljubljani, Biotehniška fakulteta, Oddelek za biologijo
Leto izdaje		2002
Obseg		str. 34
Jezik		slo
Abstrakt		Two tumor model cell lines - B16 (murine melanoma cells) and MCFlOAT (ras transformed spontaneously immortalized human breast epithelial cells) were assayed for inhibition of their invasive potential in vitro by naturally occuring inhibitors. We tested cysteine protease inhibitors from potato Solanum tuberosum var. Desiree PCPI (potato eysteine proteinase inhibitor) 6,6 in PCPI 8,7, clitocypin from mushroom Clilocybe nebularis and equistatin from sea anemone Actinia eguina. Matrigel coated filters were used to simulate the basement membrane. MTT assay was used for quantification of invading cells. B16 invasion in vitro was significantly lower in the presence of PCPI 8,7, clitocypin on the other hand increased the invasive potential of MCFIOAT. PCPI 6,6 and equistatin showed no effect in non-citotoxic concentration range. The specific activities of two intracellular cysteine proteases, catepsin B and catepsin L were measured in the same inhibitor concentration range. More than two orders of magnitude higher concentrations compared to inhibition constants from literature were efficient in inhibiting cathepsin L-like activity at physiological conditions. Comparing cathepsin L-like specific activity we found no correlation with invasive potentials of both cell lines.
Deskriptorji		TUMOR CELLS, CULTURED NEOPLASM INVASIVENESS CYSTEINE PROTEINASE INHIBITORS MELANOMA, EXPERIMENTAL POTATOES MYCOTOXINS CNIDARIAN VENOMS