Avtor/Urednik		Meneghel-Rozzo, Tiziana; Rozzo, Aldo; Poppi, Lisa; Rupnik, Marjan
Naslov		In vivo and in vitro development of mouse pacreatic beta-cells in organotypic slices
Tip		članek
Vir		Cell Tissue Res
Vol. in št.		Letnik 316
Leto izdaje		2004
Obseg		str. 295-303
Jezik		eng
Abstrakt		Taking tissue slices of the embryonic and newborn pancreas is a novel approach for the study of the perinatal development of this gland. The aim of this study was to describe the morphology and physiology of in vivo and in vitro developing beta-cells. In addition, we wanted to lay a foundation for the functional analysis of other pancreatic cells, either alone or as part of an integrative pancreatic physiology approach. We used cytochemistry and light microscopy to detect specific markers and the whole-cell patch-clamp to assess the function of single beta-cells. The insulin signal in the embryonic beta-cells was condensed to a subcellular compartment and redistributed throughout the cytosol during the first 2 days after birth. The hormone distribution correlated well with the development of membrane excitability and horrnone release competence in beta-cells. Endocrine cells survived in the organotypic tissue culture and maintained their physiological properties for weeks. We conclude that our preparation fulfills the criteria for a method of choice to characterize the function of developing pancreas in wildtype and genetically modified mice that die at birth. We suggest organotypic culture for in vitro studies of the development and regeneration of beta-cells.
Deskriptorji		ISLETS OF LANGERHANS INSULIN GLUCOSE KERATIN MEMBRANE POTENTIALS ANIMALS, NEWBORN BIOLOGICAL MARKERS CELL COMPARTMENTATION CELL DIFFERENTIATION CELL SURVIVAL CYTOSOL GANGLIA, AUTONOMIC IMMUNOHISTOCHEMISTRY MICE ORGAN CULTURE PATCH-CLAMP TECHNIQUES POTASSIUM CHANNELS TAU PROTEINS