Avtor/Urednik		Hajdinjak, Tine
Naslov		Nekateri polimorfizmi posameznih nukleotidov pri sporadičnem raku prostate
Tip		monografija
Kraj izdaje		Ljubljana
Založnik		Univerza v Ljubljani, Medicinska fakulteta
Leto izdaje		2005
Obseg		str. 55
Jezik		slo
Abstrakt		Background. The most important risk factor for developing clinical prostate cancer (PCa) is heredity. As it was shown that multiple genes, each having low penetrance, may be responsible for most inherited prostate cancer susceptibility, single nucleotide polymorphisms (SNP) are expected among new prognostic factors. Products of different genes influence prostate cancer, among them: UGT2B15 (metabolism of dihydrotestosterone), CDHI (E-cadherin - adhesion and polarisation of epithelia), GNB3 (G proteins - growth regulation), TGFBI (TGFB I - regulation of differentiation), CTSB (cathepsin B - extracellular matrix degradation). Methods. The study evaluated possible role of 6 SNPs in PCa (DBSY in gene UGT2B15, -160C/A in gene CDHI, L26V and Ex12+419G/C in gene CTSB, C82ST in gene GNB3 and L10P in gene TGFBI). With two methods for genotyping (restriction fragment lenght polymorphism and TaqMan), after tests were developed, frequencies of alleles were assessed among patients and samles from general population. When differences were noted, further analysis was performend in subgroups of patients with PCa according to stage, Gleason score, age at diagnosis and survival. Results. Frequencies of SNPs among PCa patients and general Slovenian, Caucasian population in brackets: D85Y 47/206 DD, 101/206 DY (28/178 DD, 92/178 DY), -160 C/A 21/183 AA, 72/183 AC (12/198 AA, 81/198 AC); C825T 35/83 CC, 36/83 CT (92/200 CC, 92/200 CT), L10P 12/94 CC, 51/94 CT (34/200 CC, 98/200 CT); Ex12+419G/C 48/98 GG, 45/98 GC (55/118 GG, 51/118 GC) in L26V 82/168 VV, 63/168 LV (60/168 VV, 82/168 LV). (Abstract truncated at 2000 characters)
Deskriptorji		PROSTATIC NEOPLASMS POLYMORPHISM (GENETICS) STANOLONE CADHERINS CATHEPSIN B GTP-BINDING PROTEINS GENOTYPE POLYMORPHISM, RESTRICTION FRAGMENT LENGTH POLYMERASE CHAIN REACTION ALLELES TRANSFORMING GROWTH FACTOR BETA