Avtor/Urednik		Božič, B; Čučnik, S; Kveder, T; Rozman, B
Naslov		Avidity of anti-beta-2-glycoprotein I antibodies
Tip		članek
Vir		Autoimmunity reviews
Vol. in št.		Letnik 4
Leto izdaje		2005
Obseg		str. 303-8
Jezik		eng
Abstrakt		The terms affinity and avidity are often used indiscriminately, despite clearly differing. Since affinity refers to monovalent binding of antibodies to a monovalent epitope, the majority of data on the binding of anti-beta2-glycoprotein I antibodies (anti-beta2-GPI) characterized their avidity rather than affinity. Anti-beta2-GPI were generally believed to be of low avidity, but heterogeneous avidity of patients' IgG anti-beta2-GPl has been demonstrated. High avidity anti-beta2-GPI monoclonals were reported to possess higher pathogenicity than low avidity anti-beta2-GPI. Polyclonal high avidity anti-beta2-GPI were found to be more common in patients with antiphospholipid syndrome (APS) and associated with thrombosis. Some conformational changes of beta2-GPI are required for the binding of polyclonal anti-beta2-GPI to the antigen: neither high density of the antigen nor high avidity of the anti-beta2-GPI alone is sufficient for the recognition. Avidity of anti-beta2-GPI should be considered in any attempt of inter-laboratory standardisation and/or evaluation of anti-beta2-GPl enzymelinked immunosorbent assay (ELISA).
Deskriptorji		ANTIPHOSPHOLIPID SYNDROME LUPUS ERYTHEMATOSUS, SYSTEMIC AUTOANTIBODIES GLYCOPROTEINS ANTIBODY AFFINITY ANTIBODIES, MONOCLONAL IGG ENZYME-LINKED IMMUNOSORBENT ASSAY