| Avtor/Urednik | Avguštin, Bojana; Kotnik, Vladimir; Škoberne, Mojca; Malovrh, Tadej; Skralovnik-Štern, Aleksandra; Terčelj, Marjeta | |
| Naslov | CD69 expression on CD4+ T lymphocytes after in vitro stimulation with tuberculin is an indicator of immune sensitization against Mycobacterium tuberculosis antigens | |
| Tip | članek | |
| Vir | Clin Diagn Lab Immunol | |
| Vol. in št. | Letnik 12, št. 1 | |
| Leto izdaje | 2005 | |
| Obseg | str. 101-6 | |
| Jezik | eng | |
| Abstrakt | The expression of the CD69 antigen on CD4 T lymphocytes after in vitro stimulation with purified protein derivative (2 tuberculin units) was used to evaluate the tuberculin reactivities of 52 individuals from four experimental groups: Mycobacterium bovis BCG-vaccinated healthy individuals with a negative tuberculin skin test (TST) result (group A), BCG-vaccinated healthy individuals with a positive TST result (group B), patients with active tuberculosis (TB) before treatment (group C), and individuals with clinically inactive TB who had previously completed a prescribed course of chemotherapy (group D). The expression of CD69 on CD4 T lymphocytes was significantly higher in patients with active TB (16.2%+/-7.3%), individuals with clinically inactive TB (10.5%+/-7.4%), and healthy individuals with a positive TST result (15.5%+/-7.2%) than in healthy individuals with a negative TST result (3.8%+/-4.3%) (P<0.005). We confirmed the correlation between CD69 antigen expression on T lymphocytes after stimulation with tuberculin and the TST induration diameter (Spearman rho=0.783; P<0.001), an assay for gamma interferon (the Quantiferon-TB assay; Spearman rho=0.613; P<0.001), and the lymphocyte BLAST transformation test (Spearman rho=0.537; P<0.001). Our results demonstrate the usefulness of the determination of CD69 on CD4 T lymphocytes after in vitro stimulation with tuberculin as a rapid indicator of immune sensitization against Mycobacterium tuberculosis. | |
| Deskriptorji | MYCOBACTERIUM TUBERCULOSIS TUBERCULIN BCG VACCINE CD4-POSITIVE T-LYMPHOCYTES ANTIGENS, DIFFERENTIATION, T-LYMPHOCYTE AGE FACTORS TUBERCULOSIS SEX FACTORS FLOW CYTOMETRY ANTIGENS, CD ANTIGENS, BACTERIAL |