Avtor/Urednik		Radochova, Barbora; Janaček, Jiri; Schwarzerova, Katerina; Demjenova, Erna; Tomori, Zoltan; Karen, Petr; Kubinova, Lucie
Naslov		Analysis of endoplasmic reticulum of tobacco cells using confocal microscopy
Tip		članek
Vir		Image Anal Stereol
Vol. in št.		Letnik 24, št. 3
Leto izdaje		2005
Obseg		str. 181-5
Jezik		eng
Abstrakt		Image analysis techniques for preprocessing, segmentation and estimation of geometrical characteristics of fiber-like structures from 2-D or 3-D images captured by a confocal microscope are presented. Methods are demonstrated on fiber-like biological structure: endoplasmic reticulum (ER) of tobacco cells. In the presented analysis of 2-D images of ER before and after the treatment of latrunculin B, ER and ER tubules were segmented and the area density of ER as well as the length density of ER tubules in the cell cortical layer were estimated by automatic image analysis algorithms. Images of 3-D arrangement of ER were reconstructed and rendered by various visualization techniques.
Deskriptorji		TOBACCO ENDOPLASMIC RETICULUM MICROSCOPY, CONFOCAL IMAGE PROCESSING, COMPUTER-ASSISTED