Avtor/Urednik		Kokalj-Vokač, Nadja; Zagorac, Andreja; Erjavec, Alenka; Zagradišnik, Boris
Naslov		Molecular cytogenetic technologies in cancer diagnostics
Prevedeni naslov		Molekularne citogenetske tehnike pri diagnostiki raka
Tip		članek
Vir		In: Luzar B, Poljak M, Glavač D, et al, editors. Molekularna diagnostika v medicini. Zbornik 15. spominsko srečanje akademika Janeza Milčinskega, 36. memorialni sestanek profesorja Janeza Plečnika, 1. srečanje Slovenskega društva za humano genetiko z mednarodno udeležbo; 2005 30 nov - 2 dec; Ljubljana. Ljubljana: Medicinska fakulteta,
Leto izdaje		2005
Obseg		str. 141-9
Jezik		eng
Abstrakt		Molecular cytogenetics has played a pivotal role in cancer genetics, both as an aid to diagnosis and in identifying recurrent chromosomal rearrangements, an essential prerequisite to identifying genes involved in cancer pathogenesis. In the late 1980's, a series of technologies based around fluorescence in situ hybridization (FISH) revolutionized the field. Interphase FISH, multicolour FISH (M-FISH, SKY) and comparative genomic hybridization (CGH) have emerged as the most significant of these. More recently, micro array technologies have come to prominence. Digital karyotyping and array-based technologies including array-CGH and oligonucleotide micro-array analysis have been developed recently to study the genomic landscape in human cancer. In the Laboratory of Medical Genetics, Maribor Teaching Hospital, we have developed all the basic molecular cytogenetics techniques with the exception of chip technologies, in order to study and perform diagnostics in leukemias, lymphomas and some solid tumours. In this paper the use of FISH techniques, interphase-FISH. M-FISH and CGH, and some diagnostic cases of leukemia are presented. Diagnostic cases of Her2 amplifications in breast cancer and interphase-FISH of recurrent chromosome rearrangements in bladder cancer cells are also discussed.
Deskriptorji		NEOPLASMS LEUKEMIA IN SITU HYBRIDIZATION, FLUORESCENCE KARYOTYPING PHENOTYPE