Avtor/Urednik		Poljak, Mario
Naslov		Molecular diagnostic microbiology - first twenty years
Prevedeni naslov		Molekularna diagnostična mikrobiologija - prvih 20 let
Tip		članek
Vir		In: Luzar B, Poljak M, Glavač D, et al, editors. Molekularna diagnostika v medicini. Zbornik 15. spominsko srečanje akademika Janeza Milčinskega, 36. memorialni sestanek profesorja Janeza Plečnika, 1. srečanje Slovenskega društva za humano genetiko z mednarodno udeležbo; 2005 30 nov - 2 dec; Ljubljana. Ljubljana: Medicinska fakulteta,
Leto izdaje		2005
Obseg		str. 331-41
Jezik		eng
Abstrakt		In the last two decades, molecular methods had a dramatic impact on our ability to understand epidemiology, natural history and pathogenesis of many infectious diseases. These methods are today indispensable in (i) detecting the microorganisms that are otherwise difficult or impossible to detect, identify and characterize, (ii) assessing the stage, progression, prognosis and monitoring of some important (mainly viral) infectious diseases and (iii) monitoring the efficacy of various antimicrobial therapeutic interventions. In the last two decades, the-molecular techniques which we have used in clinical microbiology have depended mainly on the use of nucleic acid probes or different target or signal amplification techniques. Polymerase chain reaction (PCR) still remains the most widely used molecular method in both research and diagnostic microbiology laboratories. Other amplification techniques like, ligase chain reaction, strand displacement amplification, branched DNA (bDNA), and nucleic acid sequence-based amplification group of methods have also been widely used in diagnostic clinical microbiology laboratories in the last decade. In addition, several exciting and very promising technologies, like DNA arrays, microarrays, biosensors, and lab-on-chip have emerged recently. Whether their performance will match the present diagnostic molecular assays in terms of sensitivity, specificity as well as costs remains to be seen.
Deskriptorji		NUCLEIC ACID HYBRIDIZATION POLYMERASE CHAIN REACTION MICROBIOLOGICAL TECHNIQUES BIOSENSORS