Avtor/Urednik		Žolnir-Dovč, Manca; Eržen, Damijan
Naslov		Molecular diagnostics of tuberculosis - progress but no gold standard
Prevedeni naslov		Molekularna diagnostika tuberkoloze - napredek, vendar ne "zlati" standard
Tip		članek
Vir		In: Luzar B, Poljak M, Glavač D, et al, editors. Molekularna diagnostika v medicini. Zbornik 15. spominsko srečanje akademika Janeza Milčinskega, 36. memorialni sestanek profesorja Janeza Plečnika, 1. srečanje Slovenskega društva za humano genetiko z mednarodno udeležbo; 2005 30 nov - 2 dec; Ljubljana. Ljubljana: Medicinska fakulteta,
Leto izdaje		2005
Obseg		str. 401-8
Jezik		eng
Abstrakt		The laboratory plays a significant role in the control of tuberculosis. Rapid reporting of smears, positive culture results and results of drug susceptibility testing are essential for treatment of patients and also for minimizing the transmission of tubercle bacilli to other people. Conventional methods for laboratory diagnostics of tuberculosis have low sensitivity and specificity (microscopic examination of acid-fast bacilli) and/or are time consuming (culture, identification of mycobacteria. susceptibility testing). An important improvement in the direct detection of tubercle bacilli in clinical specimens and in the identification of mycobacteria has been achieved by the methods using nucleic acid amplification techniques during the last fifteen years. These methods have reduced the diagnostic time from weeks to days in many cases, but their sensitivity is still lower than the sensitivity of culture. In addition, the nucleic acid amplification tests may generate false positive results. Therefore their results must always be interpreted in the context of conventional microbiological methods and the patient's clinical and epidemiological data. Although the nucleic acid amplification methods represent important improvements over most conventional methods, the culture remains the gold standard in the laboratory diagnostics of tuberculosis.
Deskriptorji		TUBERCULOSIS MYCOBACTERIUM TUBERCULOSIS POLYMERASE CHAIN REACTION