Avtor/Urednik     Liang, Feng-Xia; Bosland, Maarten C; Huang, Hongying; Romih, Rok; Baptiste, Solange; Deng, Fang-Ming; Wu, Xue-Ru; Shapiro, Ellen; Sun, Tung-Tien
Naslov     Cellular basis of urothelial squamous metaplasia: roles of lineage heterogeneity and cell replacement
Tip     članek
Vir     J Cell Biol
Vol. in št.     Letnik 171, št. 5
Leto izdaje     2005
Obseg     str. 835-44
ISSN     0021-9525
Jezik     eng
Abstrakt     AIthough the epithelial lining of much of the mammalian urinary tract is known simply as the urothelium, this epithelium can be divided into at least three lineages of renal pelvis/ureter, bladder/ trigone, and proximal urethra based on their embryonic origin, uroplakin content, keratin expression pattern, in vitro growth potential, and propensity to keratinize during vitamin A deficiency. Moreover, these cells remain phenotypically distinct even after they have been serially passaged under identical culture conditions, thus ruling out loca) mesenchymal influence as the sole cause of their in vivo differences. During vitamin A deficiency, mouse urothelium form multiple keratinized foci in proximal urethra probably originating from scattered K14-positive basal cells, and the keratinized epithelium expandshorizontally to replace the surrounding normal urothelium. These data suggest that the urothelium consists of multiple cell lineages, that trigone urothelium is closely related to the urothelium covering the rest of the bladder, and that lineage heterogeneity coupled with cell migration/replacement form the cellular basis for urothelial squamous metaplasia.
Deskriptorji     UROTHELIUM
METAPLASIA
VITAMIN A DEFICIENCY
BLADDER
KIDNEY PELVIS
MICE, INBRED C57BL
CATTLE
URETHRA
CELLS, CULTURED
KERATIN
IMMUNOHISTOCHEMISTRY
FLUORESCENT ANTIBODY TECHNIQUE
PHENOTYPE
MICROSCOPY, ELECTRON