Avtor/Urednik		Schaefer, Ralf; Knauf, Ulrich; Zweyer, Margit; Hoegemeier, Oliver; de Guarrini, Francesca; Liu, Xia; Eichhorn, Heinz Juergen; Koch, Walter; Mundegar, Rustam; Eržen, Ida; Wernig, Anton
Naslov		Age dependence of the human skeletal muscle stem cell in forming muscle tissue
Tip		članek
Vir		Artif Organs
Vol. in št.		Letnik 30, št. 3
Leto izdaje		2006
Obseg		str. 130-40
Jezik		eng
Abstrakt		Human skeletal muscle stem cells from healthy donors aged 2-82 years (n=13) and from three children suffering from Duchenne Muscular Dystrophy (DMD) were implanted into soleus muscles of immunoincompetent mice and were also expanded in vitro until senescence. Growth of implanted cells was quantified by structural features and by the amount of human DNA present in a muscle. Proliferative capacity in vitro and in vivo was inversely related to age of the donor. In vitro, a decline of about two mean population doublings (MPDs) per 10 years of donors age was observed. Muscle stem cells from DMD children were prematurely aged. In general, cell preparations with low or decreasing content in desmin-positive cells produced more MPDs than age-matched high-desmin preparations and upon implantation more human DNA and more nonmyogenic than myogenic tissue. Thus, a "Desmin Factor" was derived which predicts" quality" of the human muscle tissue growing in vivo. This factor may serve as a prognostic tool.
Deskriptorji		MUSCLE, SKELETAL STEM CELLS MUSCULAR DYSTROPHY DESMIN DNA MICE CELLS, CULTURED AGE FACTORS IMMUNOHISTOCHEMISTRY IN SITU HYBRIDIZATION POLYMERASE CHAIN REACTION