Avtor/Urednik		Pristovšek, Primož; Simčič, Saša; Wraber, Branka; Urleb, Uroš
Naslov		Structure of a synthetic fragment of the lipopolysaccharide (LPS) binding protein when bound to LPS and design of a peptidic LPS inhibitor
Tip		članek
Vir		J Med Chem
Vol. in št.		Letnik 48
Leto izdaje		2005
Obseg		str. 7911-4
Jezik		eng
Abstrakt		Peptidic lipopolysaccharide (LPS) antagonists are the subject of intensive research. We report an NMR and modeling study of LBP-14 (RVQGRWKVRASFFK), a synthetic fragment of the LPS binding protein (LBP). In a mixture with LPS we observed the transferred nuclear Overhauser effect and determined the LPS-bound structure of LBP-14 that was used for docking calculations to LPS. The derived complex was used to design a peptide that displayed more than 50% increase in LPS inhibition in vitro.
Deskriptorji		LIPOPOLYSACCHARIDES ANTIBIOTICS CARRIER PROTEINS MEMBRANE GLYCOPROTEINS PEPTIDE FRAGMENTS MOLECULAR STRUCTURE TUMOR NECROSIS FACTOR LEUKOCYTES, MONONUCLEAR CELLS, CULTURED MAGNETIC RESONANCE IMAGING MODELS, MOLECULAR