Avtor/Urednik		Hudler, Petra
Naslov		Imunski odziv proti ustnim bakterijam v mišjem modelu kroničnega vnetja (bombažna past)
Prevedeni naslov		A mouse model of chronic bacterial lesions (a cotton trap) for studying immune response against oral bacteria
Tip		monografija
Kraj izdaje		Ljubljana
Založnik		Univerza v Ljubljani, Medicinska fakulteta
Leto izdaje		1999
Obseg		str. 55
Jezik		slo
Abstrakt		Granuloma formation includes an immune response in oral tissues to various microorganisms and their products. Multiple mechanisms are involved in the pathologic changes associated with chronic periapical granulomas. Exposure to various pathogens can stimulate at least two patterns of cytokine production by T helper cells. The division of T helper cells into Th1 and Th2 subsets may be due to differences in bacterial virulence and/or the nature of MHC-peptide antigen presentation to the T helper cells. Th1 cells, involved in cellular immunity, produce IL-2, TNF beta, IFN y, while Th2 cells, with roles in humoral immunity, produce IL-4, IL-5 and IL-10. The aim of our study was to compare the activation of T helper cells and their differentiation into Th1 and Th2 subsets, due to stimulation with different bacterial strains, in a mouse model of chronic bacterial lesions (a cotton trap). We have used flow cytometry to identify the presence of intracellular cytokines of activated T cells, collected from cotton traps, previously infected with different strains of bacteria and implanted subcutaneously into the back of the mice. Our data indicate that anaerobic bacteria and nocardiae induce Th1-like cytokine secretion profile in a distant part of a cotton trap, the site where cell-mediated immune response is presumably taking part. Differences in immune response against anaerobic bacteria and nocardiae as compared to streptococci are probably dependent on some particular bacterial feature (e.g. bacterial cell wall components). The development of Th1 and Th2 type cells is essential for the eradication of pathogens, but can also be responsible for various pathological disorders. Therefore, modulation of Th cell differentiation may have clinical utility in the treatment of human disease.
Deskriptorji		PERIAPICAL GRANULOMA T-LYMPHOCYTES, HELPER-INDUCER TH1 CELLS INTERLEUKIN-2 INTERLEUKIN-4 INTERFERON TYPE II STREPTOCOCCUS STAPHYLOCOCCUS AUREUS BACTEROIDES NOCARDIA MYCOBACTERIUM DISEASE MODELS, ANIMAL MICE