Avtor/Urednik		Gojkovič, Andrej; Grošelj, Mojca
Naslov		Ugotavljanje genskih sprememb pri raku želodca
Tip		monografija
Kraj izdaje		Ljubljana
Založnik		Medicinska fakulteta
Leto izdaje		1998
Obseg		str. 45
Jezik		slo
Abstrakt		BACKGROUND. Stomach cancer (gastric adenocarcinoma) is a common malignant disease in Slovenia. According to Laurent classification we distinguish between well differentiated intestinal and less differentiated diffuse type of the disease. Both types differ in the way they develop, as their sequences of genetic changes are not the same. In the present research, performed for the first time and in selected sample of Slovenian patients, we wanted to investigate allelic loss at several tumor suppressor genes (APC, p53, Rb, nm23 and DCC) and thus distinguish between the intestinal and diffuse type of gastric adenocarcinoma in our population. The aim of this work was also to introduce new and the most recent method for investigation of allelic loss. PATIENTS AND METHODS . Seventeen randomly selected frozen samples of adenocarcinomas and of surrounding non-affected tissues were obtained from 17 patients surgically treated in 1997. Polymerase chain reaction was used for multiplying microsatellite sequences related to tumor suppressor genes. PCR products were analysed with capillary electrophoresis in automatic gene analyser. After capillary electrophoresis, five sample pairs were excluded.from further analysis because of their non-informativity which was due to non-efficient amplification. RESULTS. Loss of heterozigosity was found in 17% of samples for APC, in 50% for p53, and in 100% for DCC. There were no loss of heterozigosity for Rb and nm23. The major part of loss of heterozigosity was found in intestinal, one in mixed, and none in diffuse type of adenocarcinoma. CONCLUS IONS. We introduced the most recent method for investigating allelic loss. The results of our investigation correlate with other reports in literature for APC and p53, but not for DCC, nm23 and Rb. We found differences in genetic alterations between intestinal and diffuse type of adenocarcinoma in our population. (Abstract truncated at 2000 characters)
Deskriptorji		STOMACH NEOPLASMS ADENOCARCINOMA MICROSATELLITE REPEATS POLYMERASE CHAIN REACTION