Avtor/Urednik		Pretnar-Hartman, Katarina
Naslov		Monoklonska protitelesa proti epitopni sekvenci patološke oblike prionskega proteina, uporabna v diagnostiki
Prevedeni naslov		Monoclonal antibodies against epitope sequence of pathological form of prion protein, applicable in diagnostics
Tip		monografija
Kraj izdaje		Ljubljana
Založnik		Biotehniška fakulteta
Leto izdaje		2005
Obseg		str. 124
Jezik		slo
Abstrakt		Transmissible spongiform encephalopathies (TSEs) are neurodegenerative diseases that affect humans and animals. They are fatal, because so far, no cure has been found. The proteinaceous infective particles PrPSc, a conformational isoform of the normal prion protein (PrPC), are thought to be the causative agents. All prion diseases (human and animal) have similar characteristics. The PrPSc particles can always be found in patients, occurring in high concentrations in nervous system and in very low concentrations in other tissues. Definite diagnosis is only possible post mortem, when tests can be performed on the brain tissue, where PrPSc is determined. Before death, only surrogate disease markers can be evaluated. For research on TSEs and also for the development of a new generation of diagnostic tests it is very important to set forth a new, specific tool, for identification of PrPSc, but not its normal counterpart PrPC. The antibodies, currently used in tests for infectious agent do not differentiate between PrPC and PrPSc. All methods must include PrPC deterioration step, performed predominantly with proteinase K (PK), to which PrPSc is partially resistant. Screening tests are therefore based on PrPres detection. Nevertheless, there are extensive data that confirm PK sensitive particles that are infectious. It is generally difficult to standardise tests that include enzymatic degradation. The development of a new test is not an easy task, as the amino acid sequence of both isoforms, PrPSc and PrPC, is identical, while the secondary and tertiary structures are differentt and drastically impacting the properties of the protein. (Abstract truncated at 2000 characters)
Deskriptorji		ANTIBODIES, MONOCLONAL PRIONS PRION DISEASES IMMUNIZATION HYBRIDOMAS CELL LINE IMMUNE SERA MICE, INBRED BALB C