Avtor/Urednik		Krečič-Stres, Hana
Naslov		Časovni potek izražanja genov pri odporni sorti krompirja po okužbi z virosom PVYntn
Tip		monografija
Kraj izdaje		Ljubljana
Založnik		Univerza v Ljubljani, Medicinska fakulteta
Leto izdaje		2006
Obseg		str. 138
Jezik		slo
Abstrakt		Plants respond to sues elicited by numerous abiotic and biotic factors. Plants are constantly under attack by viruses, bacteria, fungi, invertebrates and even other plants. Usually, the manage to protect themselves from those attacks. However, Potato virus Y NTN (PVY NTN) causes potato tuber necrotic ringspot disease, which has been present in Slovenia since 1988. Infection with PVY NTN not only reduces the yield more than infection with other PVY strains, but it also causes necrotic damage to the tubers, which makes affected crops unsuitable for sale. Interactions between plants and viruses have been widely studied, however due to their complexity, they have not been fully understood yet. It has been shown, that potato cultivars differ in their sensitivity to PVY NTN. The defence response of the resistant potato cultivar Sante (Solanum tuberosum L.) to primary infection with Potato virus Y NTN was studied. We investigated the differences in gene expression pattern 0.5, 6, 12 and 24 hours after inoculation (hpi). Therefore, hybridization of potato TIGR cDNA microarrays method, which allowed us to monitor the expression of approximately 11000 genes simultaneously, was introduced and optimized. Isolation of total RNA from potato leaf tissue with RNeasy Plant Mini Kit (Qiagen) was improved to increase the capacity of the kit three fold. In addition, hybridization of TIGR potato microarrays using dendrimer labelling (3DNA Array 350TM kit with Superscript II Reverse Transcriptase) performed better than indirect labelling following RNA amplification, with respect to signal and background intensities. (Abstract truncated at 2000 characters)
Deskriptorji		POTATOES POTYVIRUS GENE EXPRESSION REGULATION, PLANT RNA DNA, COMPLEMENTARY HYBRIDIZATION SPECTROPHOTOMETRY POLYMERASE CHAIN REACTION