Avtor/Urednik		Gabrijel, Mateja
Naslov		Fuzija celic v električnem polju
Tip		monografija
Kraj izdaje		Ljubljana
Založnik		Univerza v Ljubljani, Medicinska fakulteta
Leto izdaje		2006
Obseg		str. 72
Jezik		slo
Abstrakt		Dendritic cell based vaccines are considered as a promising strategy to stimulate host immunity to recognize and eliminate malignant cells. The most promising design of dendritic cell based vaccines are hybridomas, that are produced by the fusion of dendritic and tumor cells. The potential advantage that distinguishes this approach from other dendritic cell strategies is that hybridomas present known and unknown tumor antigens on their surface in context with costimulatory molecules and MHC molecules class II and I that arise from dendritic cells. Antigen presentation in such a way results in the induction of a correspondingly balanced cytotoxic and T helper cell responses. The activation of T helper cells is crucial for effective and durable antitumor immunity. Clinical trials have demonstrated that hybridomas are able to stimulate tumor specific immunity, however only a subset of patients experienced disease regression or stabilization. There are many reasons underlying the low efficiency of hybridoma tumor vaccines. One of them is the requirement for an objective quantification of hybridoma yields, which is usually obtained by flow cytometry. However, this method is relatively insensitive, therefore our aim was to develop a new confocal microscopy based technique to asses yields of hybridomas. With this new approach a large number of electrofused cells are rapidly and objectively analyzed. The results showed that the average percentage of hybridoma cells, analyzed with confocal microscopy was around two times smaller than the average percentage of hybrids detected with flow cytometry. This may be due to higher selectivity of confocal microscopy. Flow cytometry may detect aggregates of unfused but clustered cells as hybrids, which on the other hand are easily distinguished from hybrids in confocal microscopy examination as shown here. (Abstract truncated at 2000 characters)
Deskriptorji		CELL FUSION HYBRIDOMAS LYSOSOMES DENDRITIC CELLS PC12 CELLS CHO CELLS HYBRID CELLS MICROSCOPY, CONFOCAL FLUORESCENT DYES FLOW CYTOMETRY