Avtor/Urednik		Cankar, Katarina
Naslov		Razvoj visoko zmogljivih molekularnih metod za analizo gensko spremenjenih rastlin
Tip		monografija
Kraj izdaje		Ljubljana
Založnik		Univerza v Ljubljani, Medicinska fakulteta
Leto izdaje		2006
Obseg		str. 161
Jezik		slo
Abstrakt		In a decade of commercial use of genetically modified organisms (GMOs) the global area of c biotech crops increased to 90 million hectares in 2005, and the area sown continues to increase. Due to low public acceptance of GMOs in many countries, several governments have implemented, or are in the process of adopting, legislation that requires traceability of genetically modified (GM) components and labelling of products that contain GMOs above a certain threshold. Therefore appropriate molecular techniques that enable detection, identification and quantification of genetically modified organisms, as well as new tools to evaluate safety of GM foods, have to be developed and implemented. The first step in GMO detection are screening tests, that allow for detection of GMO presence in the sample. Screening of samples is predominantly performed by detection of p35S promoter from Cauliflower mosaic virus, which has been introduced into the majority of GM plants (Holst-Jensen et al., 2003). In virus infected plants false positive results can occur (Wolf et al., 2002). A system for real-time PCR was designed that allows recognition of virus coat protein in the sample, thus allowing us to differentiate between a transgenic plant and a virus-infected plant. The developed assay has a high dynamic range of quantitative analysis and high and consistent PCR efficiency. The detection system was tested using 8 different CaMV virus isolates. No cross-reactivity was detected with the DNA of closely related Carnation etched ring virus. Primers do not amplify plant DNA neither from approved GM plants nor from different species of Brassicaceae or Solanaceae that are natural hosts of CaMV. We have evaluated the assay for different food matrices by spiking CaMV DNA into DNA from food samples and have successfully amplified CaMV from all samples. (Abstract truncated at 2000 characters)
Deskriptorji		CROPS, AGRICULTURAL PLANTS, TRANSGENIC PLASMIDS POLYMERASE CHAIN REACTION OLIGONUCLEOTIDE PROBES NUCLEIC ACIDS DNA RNA CAULIMOVIRUS BRASSICA