Avtor/Urednik		Prelovšek, Petra-Maja
Naslov		Morfološke in funkcionalne značilnosti hepatocitov močerilarjev (Amphibia: Proteidae) v primarni kulturi
Tip		monografija
Kraj izdaje		Ljubljana
Založnik		Univerza v Ljubljani, Medicinska fakulteta
Leto izdaje		2006
Obseg		str. 127
Jezik		slo
Abstrakt		Primary cultures of hepatocytes are a well established system for the study of many aspects of hepatic biology. Furthermore, isolated hcpatocytes are used for pharmacological and toxicological studies, and as therapeutic tools. Among the poikilotherms, fish are the most studied organism. Primary cultures of fish hepatocytes are used for metabolical and ecotoxicological studies and surve as a model for comparative interspecies studies. Little work, however, has been directed toward isolation and characterization of primary amphibian hepatocytes. Cultured amphibian hepatocytes are potentially useful as a model in comparative metabolical studies. Since environmental pollution is one of the factors responsible for the world-wide disappearance of amphibians, cultured amphibian hepatocytes could also serve as a model in environmental toxicology. The purpose of the present study was to isolate and to describe certain morphological and functional characteristics of amphibian hepatocytes in primary culture. Hepatocytes were isolated from two amphibian species: the common mudpuppy (Necturus maculosus) and the cave salamander (Proteus anguinus). We examined the influence of different culture conditions on hepatocyte viability and described hepatocyte ultrastructural and functional characteristics during culturing. The functional integrity of hepatocytes was estimated by tyrosine aminotransferase (TAT) induction with hydrocortisone. The isolation of N. maculosus hepatoeytes described in this paper is a modification of the well-established two-step perfusion method. A typical perfusion yielded 1.82X105 viable hepatocytes per gram body weight with an average viability of 86 +- 5%. Isolated hepatocytes retained most ultrastructural characteristics as in vivo. The volume of the cells and nuclei was slightly lower, and the amount of heterochromatin higher, compared to the conditions in vivo. (Abstract truncated at 2000 characters)
Deskriptorji		PROTEIDAE CELLS, CULTURED LIVER HYDROCORTISONE TYROSINE TRANSAMINASE MICROSCOPY, ELECTRON CULTURE MEDIA CELL SURVIVAL