Avtor/Urednik		Felber, B; Felber, E; Franz, S; Ruzicka, T; Meurer, M
Naslov		Antibodies to the 46 KD LA/SSB protein detected by immunoblotting: their clinical relevance in patients with connective tissue diseases
Tip		članek
Vir		Acta Dermatovenerol Alp Panon Adriat
Vol. in št.		Letnik 1, št. 1
Leto izdaje		1992
Obseg		str. 3-8
Jezik		eng
Abstrakt		The clinical relevance of antibodies against La/SSB was investigated in patients with systemic lupus erythematosus (SLE), subacute cutaneous lupus erythematosus (SCLE), discoid lupus erythematosus (DLE), mixed connective tissue disease (MCTD) and systemic scleroderma (PSS). La/SSB antibodies were detected by two methods: immunodiffusion against soluble rabbit thymus extract, and immunoblotting using a cytoplasmic HeLa cell extract as antigen source. Both methods had an equal sensitivity, however by immunoblotting it was possible to differentiate the autoimmune response against different polypeptides. By immunoblotting La/SSB positive sera were shown to react with a 46 kD protein and an additional 43 kD degradation fragment. Antibodies to these La/SSB proteins were found in the sera of 11 of 37 patients with SLE (30 per cent ), 9 of 23 patients with SCLE (39 per cent ),1 of 12 patients with MCTD (85),1 of 56 patients with PSS (2 per cent ), but in no serum of patients with DLE (0/14). The clinical comparison of patients with or without antibody against the 46 kD La/SSB protein indicates that in SLE patients the presence of anti-La/SSB is associated with a more benign course of the disease. At the present state of investigation, the La/ SSB antibody system showed no correlation with specific clinical findings of the other diseases studies, including SCLE.
Deskriptorji		CONNECTIVE TISSUE DISEASES ANTIBODIES, ANTINUCLEAR IMMUNOBLOTTING FLUORESCENT ANTIBODY TECHNIQUE