Avtor/Urednik		Bavec, Aljoša; Jiang, Yang; Langel, Ulo; Zorko, Matjaž
Naslov		Role of cysteine 341 and arginine 348 of GLP-1 receptor in G-protein coupling
Tip		članek
Vir		Mol Biol Rep
Vol. in št.		Letnik 34, št. 1
Leto izdaje		2007
Obseg		str. 53-60
Jezik		eng
Abstrakt		We have demonstrated the ability of peptides derived from the third intracellular loop of GLP-1 receptor to differently modulate activity of four different types of G-proteins overexpressed in sf9 cells. In this respect, the involvement of Cys(341) in inhibition of G(s) and Cys(341) in activation of G(s) and in inhibition of G(i1,) G(o), and G(11), respectively, indicates their potential role in discrimination between different types of G-proteins. Moreover, these two amino acids from the third intracellular loop might represent an important novel targets for covalent modification by downstream regulators in signaling through GLP-1 receptor.
Deskriptorji		GTP-BINDING PROTEINS RECEPTORS, GLUCAGON CYSTEINE ARGININE RECOMBINANT PROTEINS CELLS, CULTURED IMMUNOBLOTTING