Avtor/Urednik		Kovačevič, Katerina; Budefeld, Tomaž; Majdič, Gregor
Naslov		Reduced seminiferous tubule diameter in mice neonatally exposed to perfume
Prevedeni naslov		Zmanjšan premer semenskih cevk pri miših, izpostavljenih parfumu ob rojstvu do odstavitve
Tip		članek
Vir		Slov Vet Res
Vol. in št.		Letnik 43, št. 4
Leto izdaje		2006
Obseg		str. 177-83
Jezik		eng
Abstrakt		Odors play important roles in rodent social behavior and reproduction. Pheromones, acting through vomeronasal organ, influence pubertal development, estrus cycling and implantation in female mice. Therefore, we examined whether constant exposure to strong odor (perfume) could influence postnatal development of male reproductive organs. A breeding pairs of mice (one male and one female) were constantly exposed to perfume in cages with filter tops for one week before mating, throughout pregnancy and until weaning of the pups. No difference was observed in litter size or time between pregnancies. Male pups were sacrificed at 6, 16, 19 and 50 days of life and their testes fixed in Bouin's solution. Separate group of sexually mature (60 days old) male mice was exposed to perfume for 12 days and blood collected on 4, 8 and 12 day of exposure. Immunohistochemical staining using antibodies against 3J3-HSD, antimullerian hormone and proliferating cell nuclear antigen did not reveal major differences in postnatal development of testicular cells. Testosterone levels in blood collected from sexually mature mice were lower in a group exposed to perfume in comparison to control group, but difference was not statistically significant. However, seminiferous tubule diameter was reduced (p<0.05) in group of male mice exposed perinataly to perfume in comparison to control group. Although the present study did not reveal a major effect of constant exposure perfume on reproductive function in mice, reduced tubule diameter could suggest moderate changes in hormone levels such as FSH, which is known to influence Sertoli cell proliferation.
Izvleček		Vonji imajo pri glodavcih zelo pomembno vlogo pri socialnem obnašanju in obnašanju, povezanem z razmnoževanjem. Feromoni, ki jih organizem zaznava z vomeronazalnim organom, vplivajo na začetek pubertete,delovanje spolnega ciklusa in vsaditev zarodkov pri miših. Zaradi pomembne vloge vonjev za delovanje spolnega sistema smo v opisani raziskavi ugotavljali, ali lahko stalna izpostavljenost močnemu vonju, ki ga oddaja parfum, vpliva na razvoj moških spolnih organov po rojstvu. Pari miši (en samec in ena samica) so bili stalno izpostavljeni parfumu v kletkah s filtrskimi pokrovi, in sicer en teden pred parjenjem, med parjenjem in do odstavitve mladičev. Med kontrolno (skupina brez parfuma) in poskusno skupino ni bilo razlik v številu živorojenih mladičev. Moške potomce smo žrtvovali na 6., 16., 19. in 50. dan starosti in njihova moda učvrstili v bouinovem učvrščevalcu. Posebna skupina spolno zrelih živali (starih 60 dni) je bila izpostavijena parfumu 12 dni, v tem času pa smo jim 4., 8., in 12. dan odvzeli kri. Imunohistokemično barvanje z uporabo protiteles proti antimulerjevemu hormonu, 3beta-hidroksi steroidni dehidrogenazi in antigenu delečih se celic ni pokazalo nobenih očitnih razlik v razvoju mod med kontrolno in poskusno skupino. Raven testosterona je bila v skupini, izpostavljeni vplivu parfuma sicer nižja kot v kontrolni skupini, vendar pa razlika ni bila statistično značilna. Ugotovili pa smo statistično značilno razliko v premeru semenskih cevk pri 50 dni starih živalih. Premer je bil manjši (p<0.05) pri samcih, ki so bili v obdobju po rojstvu izpostavljeni vplivu parfuma v primerjavi s samci iz kontrolne skupine. (Izvleček skrajšan na 2000 znakov)
Deskriptorji		PHEROMONES PERFUME TESTIS SEMINIFEROUS TUBULES TESTOSTERONE IMMUNOHISTOCHEMISTRY MICE, INBRED BALB C