Avtor/Urednik     Milani, Marziale; Drobne, Damjana; Tatti, Francesco
Naslov     FIB/SEM for biological data mining: foused ion beam (FIB) cell sectioning for X-ray analysis - part 1
Tip     članek
Vir     Imaging Microsc
Vol. in št.     Letnik 2
Leto izdaje     2007
Obseg     str. 2-4
Jezik     eng
Abstrakt     Focused Ion Beams (FIBs) provide a cross-sedioning tool for submicron dissection of organs, tissues, cells and subcellular structures of prepared (i.e. chemically fixed) samples. In combination with a Scanning Electron Microscope (SEM), FIB makes electron and ion microscopy available at the same time, for complementary morphological information, that moreover can be completed by EDS (Energy Dispersive X-ray Spectroscopy). The case of metal granules in P. scaber hepatopancreas is presented. Part I will be devoted to FIB sectioning and SEM imaging of the sample. Part II will be devoted to EDS analysis and it will appear on the next issue of this journal.