Avtor/Urednik		Rupnik, M; Zorec, R
Naslov		Cytosolic chloride ions stimulate Ca2+-induced exocytosis in melanotrophs
Tip		članek
Vir		Febs Lett
Vol. in št.		Letnik 303, št. 2-3
Leto izdaje		1992
Obseg		str. 221-3
Jezik		eng
Abstrakt		We used the whole-cell patch-clamp technique to study the secretory activity of single cells by monitoring changes in membrane capacitance (Neher, E. and Marty, A. (1982) Proc. Natl. Acad. Sci. USA 79, 523-535) in anterior pituitary cells. Unexpectedly we have observed that increasing intracellular chloride ions stimulate Ca2+-induced exocytosis in a dose-dependent fashion (Kd =12 mM). These results demonstrate a role of cytosolic chloride ions in the regulation of exocytotic secretion in anterior pituitary cells. It is suggested that chloride channels, in addition to playing a part in regulating membrane electrical activity (Korn, S.J., Bolden, A. and Horn, A. ( 1991 ) J. Physiol. 439 423-437; Penner, R., Matthews, G. and Horn, A. (1988) Nature 334, 499-504) and cytosolic pH (Kaila, K. and Voipio, J. (1987) Nature 330,163-165), are also involved in the modulation of cytosolic chloride concentration and thus in the control of exocytosis.
Deskriptorji		PITUITARY GLAND, ANTERIOR CHLORIDES EXOCYTOSIS RATS CELLS, CULTURED