| Avtor/Urednik | Rozman, Damjana; Juvan, Peter | |
| Naslov | DNA microarray technology in medical diagnostics | |
| Prevedeni naslov | Tehnologija DNA mikromrež v medicinski diagnostiki | |
| Tip | članek | |
| Vir | Zdrav Vestn | |
| Vol. in št. | Letnik 76, št. Suppl 2 | |
| Leto izdaje | 2007 | |
| Obseg | str. II-53-60 | |
| Jezik | eng | |
| Abstrakt | Background DNA microarray technology opened a completely new venue in the biomedical research. By allowing to follow the expression of thousands of genes in a single experiment (ideally we can follow the expression of the entire genome), the microarray technology brings new perspectives for improvement of prognostics and diagnostics of complex human diseases. By SNP arrays that allow identification of individual patient's genotype for frequent genetic diseases, this technology contributes to the efforts towards personalized medicine. Methods Different approaches to microarray technology are presented with some examples of its application in medicine and pharmacogenomics. Practical considerations for using microarrays are described, including selecting an appropriate platform, designing and performing an experiment, and managing data. Statistical issues in microarray data analysis are raised and the most common analysis techniques are listed. In conclusion the current state of microarray technology in Slovenia is addressed. | |
| Izvleček | Izhodišča Tehnologija DNA mikromrež je prinesla nov vpogled v biomedicinske raziskave. Z zmožnostjo sledenja izražanja tisočerih genov hkrati (v limiti lahko sledimo izražanje celotnega genoma), tehnologija mikromrež prinaša nove obete za izboljšanje prognoze in diagnoze kompleksnih obolenj pri človeku. Pomaga pa tudi utirati pot osebni medicini, saj je s tematskimi SNP mikromrežami možno ugotavljati genotip bolnikov za bolj znana in pogostejša genetska obolenja. Metode Predstavljeni so pristopi tehnologije mikromrež in primeri njene uporabe v medicini in furmakogenomiki. Podani so praktični napotki za uporabo mikromrež kot so izbira primerne platforme, načrtovanje in izvedba poskusa, in upravljanje s podatki. Izpostavljen je statističen pogled na analizo podatkov mikromrež in naštete so najpogosteje uporabljene metode. Trenutno stanje tehnologije mikromrež v Sloveniji je opisano v zaključku. | |
| Deskriptorji | GENOME, HUMAN GENE EXPRESSION PHARMACOGENETICS TRANSCRIPTION, GENETIC OLIGONUCLEOTIDE PROBES |