| Abstrakt | | Background. Cardiovascular disease remains the leading cause of mortality and morbidity in the western culture. Substances with anti-inflammatory and anti-oxidative action are known to improve cardioprotection. Some of these substances are present in red wine. Various studies have indicated that mild-to-moderate red wine consumption is associated with a reduced incidence cardiovascular disease. Aim. In our study we tried to assess the influence of red wine on the development of myocardial injuries caused by ischemia/reperfusion on isolated rat hearts. The effects of red wine were compared to the effects of antiarrhythmic propafenone and antithrombotic nadroparine. Hypotesis. We anticipate the red wine to significantly decrease the ischemic/reperfusion myocardial injuries, to act as effective as antiarrhythmic and to influence the EKG signal. In hearts treated with nadroparin solution, less postischemic myocardial injuries occur. Methods. Experiments were carried out on the isolated hearts of sexually mature rats of both sexes (230-330g). We isolated rat hearts according to Langendorff's method. The hearts were perfused with Krebs-Henseleit solution aerated with carbogen for 30 min and then subjected to 40 min zero-flow ischemia followed by 50 min reperfusion. The severity of myocardial damage caused by ischemia/reperfusion was assessed by post ischemic changes in the following registered parameters: coronary flow, lactate dehydrogenase release rate (LDH), left ventricular pressure, heart rate and duration of arrhythmias during reperfusion. One way ANOVA and Bonferroni post-test were used for statistical analysis, using the program Graph Pad Prism. Statistical significant difference was determined for p<0,05. (Abstract truncated at 2000 characters)
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