Avtor/Urednik		Razinger, Jaka; Drinovec, Luka; Zrimec, Alexis
Naslov		Real-time in vivo visualization of oxidative stress in duckweed (Lemna minor L.)
Tip		članek
Vir		Cent Eur J Biol Print
Vol. in št.		Letnik 2, št. 3
Leto izdaje		2007
Obseg		str. 351-63
Jezik		eng
Abstrakt		An experimental set-up which enabled non-invamn e, real-tirue reactive oxygen slx`cia;s (R09) visualization on a whole plant level was constructed. In the test organism Lemna minor L. (common duckweed), apoplastic: and symplastic oxidative stress was evaluated by exposure to menadione (50 microM), menadione (50 microM) + ascorbate (100 pM) or neither for control. Menadione (50 microM) caused a statistically significant increase in H2DCFDA fluorescence in the apoplast after GO minutes of exposure. The addition of ascorbate (100) microM) in the test medium significantly decreased apoplastic oxidative stress. 50 microM menadione caused an increase in symplastic H2DCFDA fluorescence in 57% of fonds. The exposure of L. minor plants to both menadione and ascorbate decreased the rate of fluorescence intensity acccumulation in the symplast to control levels. The method has proven to be quick and straightforward and could be applied to a range of chemicals in various physiological and toxicological plant studies. The acivantages of the set-up and different possible artefacts are discussed.
Deskriptorji		PLANTS OXIDATIVE STRESS REACTIVE OXYGEN SPECIES VITAMIN K ASCORBIC ACID FLUORESCENCE