Avtor/Urednik     Virant-Klun, Irma; Rožman, Primož; Cvjetičanin, Branko; Vogler, Andrej; Klemenc, Polona; Maličev, Elvira; Ihan, Alojz; Šinkovec, Jasna; Vrtačnik-Bokal, Eda; Tomaževič, Tomaž; Meden-Vrtovec, Helena
Naslov     Matične celice površinskega epitelija jajčnika: oogeneza in vitro?
Prevedeni naslov     Ovarian surface epithelium stem cells: oogenesis in vitro?
Tip     članek
Vir     Zdrav Vestn
Vol. in št.     Letnik 76, št. 10
Leto izdaje     2007
Obseg     str. 603-13
Jezik     slo
Abstrakt     The dogma that the total number of follicles and oocytes available for reproduction are determined at birth, and that one follicle and one oocyte are recruited from the existing pool to mature in each menstrual cycle still persists. There is an increasing experimental evidence that this might not be true. In the ovarian surface epithelium there might be nondifferentiated stem cells that might differentiate into oocytes also in adult life. In this research we aimed at isolating putative stem cells from the ovarian surface epithelium and at evaluating in vitro oogenesis in the ovarian cell culture in vitro in women without naturally present follicles and oocytes in their ovarian cortex - postmenopausal women and women with premature ovarian failure. Ovarian surface epithelium was scraped from the ovaries of 20 postmenopausal women and S women with premature ovarian failure. We tried to find putative stem cells in ovarian scraping and to confirm them by transcription markers Oct-4, Sox-2, and Nanog, and by surface antigen SSEA-4. Cell culture was set up by scraped cells in DMEM/F-12 medium with phenol red, which shows a weak estrogenic activity. Ovarian cell culture was cultured for 20 days in a CO2 incubator at 3.7 degreesC and 5% COz. Development of cells in the culture was followed and th epresence of oocyte-like cells was evaluated by using different methods - evaluation of morphology, transcription markers, surface antigen markers, oocyte immunohistochemical markers, and flow cytometry after propidium iodide staining. (Abstract truncated at 2000 characters)
Deskriptorji     OVARY
STEM CELLS
OOCYTES
OOGENESIS
CELLS, CULTURED
POSTMENOPAUSE
IMMUNOHISTOCHEMISTRY
TRANSCRIPTION FACTORS
FLOW CYTOMETRY