Avtor/Urednik		Krašna, M; Domanovič, D; Tomšič, A; Švajger, U; Jeras, M
Naslov		Platelet gel stimulates proliferation of human dermal fibroblasts in vitro
Tip		članek
Vir		Acta Dermatovenerol Alp Pannon Adriat
Vol. in št.		Letnik 16, št. 3
Leto izdaje		2007
Obseg		str. 105-10
Jezik		eng
Abstrakt		Background: Platelet gel is a source of many soluble proteins that can modulate tissue regeneration. Dermal fibroblasts play an important role in tissue remodeling and wound healing. Objective: The study was performed to investigate the influence of platelet gel and its active ingredients on the proliferation of human dermal fibroblasts in vitro. Methods: A fibroblast culture was established from a normal human skin biopsy. Platelet gel was prepared from platelet-rich plasma (PRP) following the addition of calcified thrombin solution. cell proliferation was measured by MTT assay. Results: We showed that platelet gel, PRP, and thrombin stimulate the proliferation of dermal fibroblasts, and that stimulation by PRP is dose dependent. Conclusion: Platelet gel can be used to treat chronic skin defects.
Deskriptorji		FIBROBLASTS CELLS, CULTURED BLOOD PLATELETS CELL DIVISION