Avtor/Urednik		Devjak, Rok; Režen, Tadeja; Rozman, Damjana; Juvan, Peter
Naslov		Kontrola kvalitete DNA mikromrež nizke gostote
Prevedeni naslov		Quality control of low-density DNA microarrays
Tip		članek
Vir		Inform Med Slov Print ed
Vol. in št.		Letnik 12, št. 2
Leto izdaje		2007
Obseg		str. 1-13
Jezik		slo
Abstrakt		DNA microarray technology, showing great perspective in clinical diagnostics and prognostics, is facing a problem of loosely defined and non-unified data quality control. We have developed a general approach for controlling the quality of low density DNA microarrays, which is based on the analysis of covariance of control probes. We have applied the approach to the Steroltalk microarray, which was developed to study genes in connection with cholesterol homeostasis. We analyzed changes in genes expression in human primary hepatocytes treated with rifampicin and rosuvastatin and evaluated the approach by comparing our results to these from the literature. The proposed approach is independent of the type of DNA microarray and therefore may contribute to more rapid transfer of this technology to the clinical practice.
Izvleček		Tehnologija DNA mikromrež, ki veliko obeta na področju klinične diagnostike in prognostike, se sooča s problemom slabo izoblikovane in neenotne sheme za kontrolo kvalitete meritev. Razvili smo splošen pristop za kontrolo kvalitete DNA mikromrež nizke gostote, ki je osnovan na analizi kovariance kontrolnih sond. Pristop smo aplicirali na mikromreži Steroltalk, razviti z namenom preučevanja izražanja genov, povezanih s homeostazo holesterola. Na modelu primarnih človeških hepatocitov smo analizirali spremembe v izražanju genov pri aplikaciji riiampicina in rosuvastatina in pristop ovrednotili s primerjavo rezultatov analize z navedbami iz literature. Predlagan pristop je neodvisen od vrste uporabljene DNA mikromreže in kot tak lahko pomembno prispeva k hitrejšemu prenosu te tehnologije v klinično prakso.
Deskriptorji		OLIGONUCLEOTIDE PROBES GENE EXPRESSION CELLS, CULTURED LIVER RIFAMPIN ANTILIPEMIC AGENTS