Avtor/Urednik		Lusa, L; Cappelletti, V; Gariboldi, M; Ferrario, C; De Cecco, L; Reid, JF; Toffanin, S; Gallus, G; McShane, LM; Daidone, MG; Pierotti, MA
Naslov		Questioning the utility of pooling samples in microarray experiments with cell lines
Tip		članek
Vir		Int J Biol Markers
Vol. in št.		Letnik 21, št. 2
Leto izdaje		2006
Obseg		str. 67-73
Jezik		eng
Abstrakt		We describe a microarray experiment using the MCF-7 breast cancer cell line in two different experimental conditions for which the same number of independent pools as the number of individual samples was hybridized on Affymetrix GeneChips. Unexpectedly, when using individual samples, the number of probe sets found to be differentially expressed between treated and untreated cells was about three times greater than that found using pools. These findings indicate that pooling samples in microarray experiments where the biological variability is expected to be small might not be helpful and could even decrease one's ability to identify differentially expressed genes.