Avtor/Urednik		Trkov, Marija; Berce, Ingrid; Dovečar, Darja; Grilc, Eva; Bujko, Marina; Kraigher, Alenka
Naslov		Odkrivanje nekaterih genov, povezanih z virulenco, pri sevih E. coli, ki povzročajo črevesne okužbe
Prevedeni naslov		Detection of some virulence related genes of diarrheagenic E. coli strains
Tip		članek
Vir		Zdrav Vars
Vol. in št.		Letnik 47, št. 2
Leto izdaje		2008
Obseg		str. 81-8
Jezik		slo
Abstrakt		Aim: Identification of E. coli virulence factors improves our understanding ofthe disease they cause. Different E. coli strains isolated from patient stool samples were examined for the presence of some virulence-related genes of these bacteria, and for their ability to produce verocytotoxins VT1 and VT2. The samples were obtained from patients in the Ljubljana and Nova Gorica regions in 2006. Methods: We used the multiplex PCR (mPCR) method, which allows detection of six different virulence genes, i.e. ipaH, eltA, estA, eae,vtx1 and vtx2. The ability to produce verocytotoxins VT1 and VT2 was tested using the reverse passive latex agglutination method. Results: In six of the 42 E.coli strains examined, at least one virulence factor was identified. The presence of gene eae was confirmed in all six strains belonging to serogroups O26, O128, O145 and O157. Three intimin-positive strains were positive for vtx1 or vtx2 genes. Two strains belonged to serogroup O26 and one to serogroup O157. VT1 production was detected in one serogroup O26 strain, while one serogroup O26 strain and one serogroup O157 strain showed the ability to produce VT2. In the rest of the strains tested noverocytotoxin production was detected. All strains examined were negative for the genes ipaH, eltA and estA. Conclusion: The study showed limited circulation of E. coli strains with verocytotoxin genes and of strains able toproduce verocytotoxins in the Nova Gorica and Ljubljana regions. In order toget a larger-scale estimate, a larger number of strains from different geographical regions of Slovenia will have to be tested, and research will have to be expanded to include identification of other potential virulence factors.
Izvleček		Namen dela: Odkrivanje virulentnih dejavnikov E. coli omogoča boljše poznavanje bolezni, ki jo povzročajo. Zato smo preiskali določeno število sevov E. coli, osamljenih iz vzorcev iztrebkov bolnikov, na prisotnost nekaterih genov, povezanih z virulenco teh bakterij in ugotavljali sposobnost izdelave verocitotoksinov VT1 in VT2. Preiskovane seve so osamili v letu 2006 v ljubljanski in novogoriški regiji. Metode: Z metodo "multipli" PCR (mPCR) smo odkrivali naslednjih šest genov, povezanih z virulenco E. coli: ipaH, eltA, estA, eae, vtx1 in vtx2. Sposobnost izdelave verocitotoksinov VT1 in VT2smo ugotavljali z reverzno pasivno aglutinacijo lateksa. Rezultati: Pri šestih od 42 preiskovanih sevov E. coli smo našli vsaj en dejavnik, povezan z virulenco. Pri vseh smo ugotovili prisotnost gena za intimin (eae), pripadali pa so naslednjim serološkim skupinam: O26, O128, O145 in O157. Pri treh sevih,pri katerih smo ugotovili prisotnost gena za intimin, smo ugotovili tudiprisotnost genov za verotoksine (vtx1 ali vtx2). Dva med njimi sta pripadala serološki skupini O26, eden pa O157. En sev serološke skupine O26 jeizdeloval VT1, en sev O26 in en sev O157 pa sta izdelovala VT2. Pri drugih preiskovanih sevih nismo ugotovili sposobnosti izdelovanja omenjenih toksinov.Prisotnosti genov ipaH, eltA in estA nismo ugotovili pri nobenem preiskovanem sevu. Zaključek: S to raziskavo smo ugotovili, da v novogoriški in ljubljanski regiji kroži dokaj nizko število sevov E. coli, ki imajo gene za verocitotoksine oziroma so jih sposobni izdelovati. Za splošno oceno bi bilo potrebno nadaljevati s preiskovanjem večjega števila sevov z različnih področij Slovenije, raziskavo pa bi bilo smiselno razširiti na odkrivanje tudidrugih virulentnih dejavnikov.