Avtor/Urednik     Trinkaus, Miha; Pregelj, Peter; Sketelj, Janez
Naslov     Reciprocal neural regulation of extrajunctional acetylcholinesterase and collagen Q in rat muscles-The role of calcineurin signaling
Tip     članek
Vir     Chem Biol Interact
Vol. in št.     Letnik 175, št. 1-3
Leto izdaje     2008
Obseg     str. 45-9
ISSN     0009-2797
Jezik     eng
Abstrakt     There are two main differences regarding acetylcholinesterase (AChE) expression in the extrajunctional regions of fast and slow rat muscles: (1) the activity of AChE catalytic subunits (G1 form) is much higher in fast than in slow muscles, and (2) the activity of the asymmetric forms of AChE (A(8) and A(12)) is quite high extrajunctionally in slow muscles but virtually absent in fast muscles. The latter is due to the absence of the expression of AChE-associated collagen Q (ColQ) in the extrajunctional regions of fast muscle fibers, in contrast to its ample expression in slow muscles. We showed that both differences are caused by different neural activation patterns of fast vs. slow muscle fibers, which determine the respective levels of mRNA of both proteins. Whereas the changes in AChE mRNA levels in fast and slow muscles, as well as the levels of ColQ mRNA levels in slow muscles, observed in response to exposing either slow or fast muscles to different muscle activation patterns, are completely reversible, the extrajunctional suppression of ColQ expression in fast muscle fibers seems to be irreversible. Calcineurin signaling pathway in muscles is activated by high-average sarcoplasmic calcium concentration resulting from tonic low-frequency muscle fiber activation pattern, typical for slow muscle fibers, but is inactive in fast muscle fibers, which are activated by infrequent high-frequency bursts of neural impulses. Application to rats of two inhibitors of calcineurin (tacrolimus-FK506 and cyclosporin A) demonstrated that the mRNA levels of both the AChE catalytic subunit and ColQ in the extrajunctional regions of the soleus muscle are regulated by the calcineurin signaling pathway, but in a reciprocal way. Under the conditions of low calcineurin activity, AChE expression is enhanced and that of ColQ is suppressed, and vice versa. (Abs. trunc. at 2000 ch.)
Deskriptorji     MUSCLE, SKELETAL
ACETYLCHOLINESTERASE
COLLAGEN
CALMODULIN-BINDING PROTEINS
NEUROMUSCULAR JUNCTION
RATS
RNA, MESSENGER
MUSCLE FIBERS, FAST-TWITCH
MUSCLE FIBERS, SLOW-TWITCH
CYCLOSPORINE