Avtor/Urednik		Šmuc, T; Lanišnik-Rižner, T
Naslov		Expression of 17beta-hydroxysteroid dehyrogenases and other estrogen-metabolizing enzymes in different cancer cell lines
Tip		članek
Vir		Chem Biol Interact
Vol. in št.		Letnik 178, št. 1-3
Leto izdaje		2009
Obseg		str. 228-233
Jezik		eng
Abstrakt		Estrogen action is regulated at the receptor level by regulation of expression of estrogen receptors, and at the pre-receptor level by interconversions between the active hormone (estradiol) and its inactive counterparts (estrone, estrone-sulfate). In peripheral tissues, estrogens can be produced via the aromatase or the sulfatase pathways. Aromatase converts androstenedione and testosterone to estrone and estradiol, respectively, and sulfatase releases estrogens from inactive sulfates, while sulfotransferase catalyzes the reverse reaction. In both pathways, 17beta-hydroxysteroid dehydrogenases (17beta-HSDs) are of paramount importance as they catalyze activation of estrone to estradiol and inactivation of estradiol to estrone. These enzymes belong to either the short-chain dehydrogenase/reductase (SDR) or the aldo-keto reductase (AKR) protein superfamilies. Differential expression of these pre-receptor regulatory enzymes can lead to high estradiol concentrations, which have been implicated in the development of different diseases. Here, we have examined gene expression levels of estrogen-metabolizing enzymes, as six SDRs (17beta-HSD types 1, 2, 4, 7, 8, 12) and one AKR (17beta-HSD type 5; AKR1C3), of aromatase, steroid sulfatase (STS) and estrogen sulfotransferase (SULT1E1), and of the alpha and beta estrogen receptors (ERs), in breast cancer (MCF-7), endometrial cancer (Ishikawa), choricarcinoma (JEG3) and liver cancer (HepG2) cell lines. After RNA isolation and cDNA synthesis, real-time PCR analyses were performed. The expression of AKR1C3 was examined also at the protein level. Our data show that in all four cancer cell lines, estradiol can be synthesized from estrone by the action of 17beta-HSD type 12, or from estrone-sulfate by sulfatase. In JEG3 and HepG2 cells, estradiol can be formed from androgens by aromatase and 17beta-HSD type 1. (Abstract truncated at 2000 characters)
Deskriptorji		TUMOR CELLS, CULTURED 17-HYDROXYSTEROID DEHYDROGENASES SULFATASES SULFOTRANSFERASES AROMATASE BREAST NEOPLASMS CHORIOCARCINOMA ENDOMETRIAL NEOPLASMS LIVER NEOPLASMS POLYMERASE CHAIN REACTION BLOTTING, WESTERN