Avtor/Urednik | Osredkar, D; Burnik-Papler, T; Pečavar, B; Kralj-Iglič, V; Kržan, M | |
Naslov | Kinetic and pharmacological properties of [(3)H]-histamine transport into cultured type 1 astrocytes from neonatal rats | |
Tip | članek | |
Vir | Inflamm Res | |
Vol. in št. | Letnik 58, št. 2 | |
Leto izdaje | 2009 | |
Obseg | str. 94-102 | |
Jezik | eng | |
Abstrakt | Objective and design: Astrocytes actively participate in the inactivation of neurotransmitters. In this work we elucidated the contribution of astrocytes in clearance of histamine, a process which has not yet been fully clarified.Methods: The characteristics of [(3)H]-histamine uptake were determined in cultured neonatal rat type 1 astrocytes and histamine-N-methyl-transferase expression was determined using RT-PCR.Results: These cells transport [(3)H]-histamine in a time- and concentration-dependent manner. The histamine clearance by astrocytes was described by a mathematical model including two processes: electrodiffusion and active transport. A further analysis of kinetic parameters of a carrier-operated transport revealed a single transport system with Michaelis constant (K(m)) of 3.5 +/- 0.8 muM and a maximal uptake rate (V(max)) of 7.9 +/- 0.3 pmol/mg protein/min. From drugs tested amitriptyline, desipramine, mepyramine and cimetidine significantly decreased [(3)H]-histamine uptake. Taken-up histamine could be metabolically degraded in cultured astrocytes, since they express mRNA for enzyme histamine-N-methyltransferase.Conclusion: Astrocytes participate in the clearance of extracellular histamine by electrodiffusion and active transport by a yet not identified carrier. Taken up histamine can be converted to tele-methylhistamine within astrocytes thus indicating the involvement of astrocytes not only in clearance but also in the inactivation of histamine. | |
Deskriptorji | ASTROCYTES HISTAMINE HISTAMINE METHYLTRANSFERASE BIOLOGICAL TRANSPORT, ACTIVE POLYMERASE CHAIN REACTION KINETICS CELLS, CULTURED ANIMALS, NEWBORN RATS, WISTAR |