| Avtor/Urednik | Dobritzsch, Doreen; Gojkovic, Zoran; Andersen, Birgit; Piškur, Jure | |
| Naslov | Crystallization and preliminary X-ray analysis of beta-alanine synthase from the yeast Saccharomyces kluyveri | |
| Tip | članek | |
| Vir | Acta Crystallogr D Biol Crystallogr | |
| Vol. in št. | Letnik 59, št. Pt 7 | |
| Leto izdaje | 2003 | |
| Obseg | str. 1267-9 | |
| Jezik | eng | |
| Abstrakt | In eukaryotes and some bacteria, the third step of reductive pyrimidine catabolism is catalyzed by beta-alanine synthase (EC 3.5.1.6). Crystals of the recombinant enzyme from the yeast Saccharomyces kluyveri were obtained using sodium citrate as a precipitant. The crystals belong to space group P2(1) (unit-cell parameters a = 117.2, b = 77.1, c = 225.5 A, beta = 95.0 degrees ) and contain four homodimers per asymmetric unit. Data were collected to 2.7 A resolution. Introduction of heavy atoms into the crystal lattice induced a different set of unit-cell parameters (a = 61.0, b = 77.9, c = 110.1 A, beta = 97.2 degrees ) in the same space group P2(1), with only one homodimer per asymmetric unit. | |
| Deskriptorji | AMIDOHYDROLASES CITRATES CRYSTALLIZATION RECOMBINANT PROTEINS SACCHAROMYCES X-RAY DIFFRACTION |