Avtor/Urednik		Kristoffersen, P; Jensen, GB; Gerdes, K; Piškur, J
Naslov		Bacterial toxin-antitoxin gene system as containment control in yeast cells
Tip		članek
Vir		Appl Environ Microbiol
Vol. in št.		Letnik 66, št. 12
Leto izdaje		2000
Obseg		str. 5524-6
Jezik		eng
Abstrakt		The potential of a bacterial toxin-antitoxin gene system for use in containment control in eukaryotes was explored. The Escherichia coli relE and relB genes were expressed in the yeast Saccharomyces cerevisiae. Expression of the relE gene was highly toxic to yeast cells. However, expression of the relB gene counteracted the effect of relE to some extent, suggesting that toxin-antitoxin interaction also occurs in S. cerevisiae. Thus, bacterial toxin-antitoxin gene systems also have potential applications in the control of cell proliferation in eukaryotic cells, especially in those industrial fermentation processes in which the escape of genetically modified cells would be considered highly risky.
Deskriptorji		GENES, BACTERIAL ANTITOXINS BACTERIAL TOXINS BASE SEQUENCE BIOTECHNOLOGY CONTAINMENT OF BIOHAZARDS DNA PRIMERS ESCHERICHIA COLI GENETIC ENGINEERING GENETIC VECTORS SACCHAROMYCES CEREVISIAE