Avtor/Urednik		Munch-Petersen, Birgitte; Knecht, Wolfgang; Lenz, Camilla; Sondergaard, Leif; Piškur, Jure
Naslov		Functional expression of a multisubstrate deoxyribonucleoside kinase from Drosophila melanogaster and its C-terminal deletion mutants
Tip		članek
Vir		J Biol Chem
Vol. in št.		Letnik 275, št. 9
Leto izdaje		2000
Obseg		str. 6673-9
Jezik		eng
Abstrakt		The occurrence of a deoxyribonucleoside kinase in Drosophila melanogaster (Dm-dNK) with remarkably broad substrate specificity has recently been indicated (Munch-Petersen, B., Piskur, J., and Sondergaard, L. (1998) J. Biol. Chem. 273, 3926-3931). To prove that the capacity to phosphorylate all four deoxyribonucleosides is in fact associated to one polypeptide chain, partially sequenced cDNA clones, originating from the Berkeley Drosophila genome sequencing project, were searched for homology with human deoxyribonucleoside kinases. The total sequence of one cDNA clone and the corresponding genomic DNA was determined and expressed in Escherichia coli as a glutathione S-transferase fusion protein. The purified and thrombin cleaved recombinant protein phosphorylated the four deoxyribonucleosides with high turnover and K(m) values similar to those of the native Dm-dNK, as well as the four ribonucleosides and many therapeutical nucleoside analogs. Dm-dNK has apparently the same origin as the mammalian kinases, thymidine kinase 2, deoxycytidine kinase, deoxyguanosine kinase, and the herpes viral thymidine kinases, but it has a unique C terminus that seems to be important for catalytic activity and specificity. The C-terminal 20 amino acids were dispensable for phosphorylation of deoxyribonucleosides but necessary for full activity with purine ribonucleosides. Removal of the C-terminal 20 amino acids increased the specific activity 2-fold, but 99% of the activity was lost after removal of the C-terminal 30 amino acids.
Deskriptorji		AMINO ACIDS ANIMALS CLONING, MOLECULAR DEOXYRIBONUCLEOSIDES DROSOPHILA MELANOGASTER ESCHERICHIA COLI KINETICS MOLECULAR SEQUENCE DATA PHOSPHORYLATION PHOSPHOTRANSFERASES (ALCOHOL GROUP ACCEPTOR) RECOMBINANT FUSION PROTEINS SEQUENCE ALIGNMENT SEQUENCE DELETION SUBSTRATE SPECIFICITY