Avtor/Urednik		Zorn, B
Naslov		Le sperme "inflammatorie": ses relations avec la fertilite
Prevedeni naslov		"Inflammatory" sperm: relationship with fertility
Tip		članek
Vir		Andrologie
Vol. in št.		Letnik 19
Leto izdaje		2009
Obseg		str. 35-44
Jezik		fre
Abstrakt		Objectives: Sperm inflammation is caused by bacterial or viral infection of the male genitourinary tract; it is often clinically asymptomatic. There is a dilemma about the causal relationship between leukocytes as markers of inflammation and poor semen quality. We were interested in sperm chang es at molecular level caused by inflammation.Material and methods: This study was based on a literature review and personal data. In 200 male partners of infertile couples with normal semen analysis, the percentage of sperm with DNA denaturation and the level of reactive oxygen species (RaS) were determined by flow cytometric analysis, after acridine orange and dihydroethidium stainings, and correlated with seminal plasma elastase leveis. Results: In the literature, a positive relationship between inflammation and increased sperm apoptosis was found with increased necrosis and decreased mitochondrial membrane potential. We found a positive correlation between the percentage of sperm with denatured DNA and elastase leveis. The percentage increased from 8.6% at elastase level 0-100 /J.g/l to 15.7% at elastase level 100250 /J.g/l; this increase was not dependent on RaS production. The percentage of sperm with denatured DNA normalized at elastase level s above 600 /J.g/l. Discussion and conclusion: Changes in sperm DNA or membrane s do not necessarily affect classical semen characteristics or reduce fertility in males. They can, however, have anegative effect on capacitation and acrosomal reaction, resulting in failed fertilization or poor embryo development. Before treatment, we must take into account the location and the duration of the inflammation as well as the damage done to sperm.
Deskriptorji		INFERTILITY, MALE SEMEN LEUKOCYTES REACTIVE OXYGEN SPECIES DNA FLOW CYTOMETRY INFLAMMATION MITOCHONDRIA