Avtor/Urednik | Sikdar, SK; Zorec, R; Mason, WT | |
Naslov | cAMP directly facilitates Ca-induced exocytosis in bovine lactotrophs | |
Tip | članek | |
Vir | FEBS Lett | |
Vol. in št. | Letnik 273, št. 1-2 | |
Leto izdaje | 1990 | |
Obseg | str. 150-4 | |
Jezik | eng | |
Abstrakt | We have used the whole cell patch clamp technique on single prolactin-secreting bovine lactotrophs to measure plasma membrane capacitance (Cm), an index of membrane surface area, under voltage-clamp during cytosol dialysis with Ca and cAMP. cAMP increased the magnitude and rate of Ca-induced exocytosis (Cm increase) without affecting membrane conductance; however, cAMP had no detectable effect on Cm when intracellular Ca was low. We thus report new evidence that cAMP can facilitate Ca-induced secretion in a synergistic fashion, by acting directly on the secretory apparatus, independently of membrane conductance activation. | |
Deskriptorji | CALCIUM CYCLIC AMP EXOCYTOSIS PITUITARY GLAND, ANTERIOR PROLACTIN CALCIUM CATTLE CELL MEMBRANE CELLS, CULTURED EGTAZIC ACID ELECTRIC CONDUCTIVITY KINETICS MEMBRANE POTENTIALS PITUITARY GLAND, ANTERIOR |