Avtor/Urednik		Kapović, Miljenko; Rukavina, Daniel
Naslov		Reactivity to alloantigens and polyclonal mitogens and CD4+/CD8+ cell ratio shifts of cervical lymph node and spleen cells during pregnancy in rats
Tip		članek
Vir		J Reprod Immunol
Vol. in št.		Letnik 20, št. 2
Leto izdaje		1991
Obseg		str. 165-74
Jezik		eng
Abstrakt		Cervical lymph node (CLN) cells and spleen cells were harvested from virgin and pregnant rats bearing syngeneic or allogeneic fetuses at all stages of pregnancy including the pre-implantation period. The specific and non-specific alloreactivity of these cells were analyzed in MLR against mitomycin-C treated paternal strain or unrelated cells. Mitogen stimulation of the cell cultures utilized PHA, Con-A and PWM. Cells bearing T cell markers were labeled in an indirect assay using the monoclonal antibodies W3/25 and MRC OX 8. Specific alloreactivity is enhanced at mid-pregnancy in both cell populations. Non-specific alloreactivity was suppressed in the cervical lymph node cells. Spleen cells demonstrated an increased non-specific alloreactivity and T polyclonal mitogen reactivity (PHA and Con-A) at mid-pregnancy. Reactivity to Con-A was depressed in the early phase and at the end of allogeneic pregnancy in the CLN. The CD4+/CD8+ ratio was very low during all phases of pregnancy.
Deskriptorji		ANIMALS ANTIGENS, CD4 ANTIGENS, CD8 CELL DIVISION CERVIX UTERI CONCANAVALIN A IMMUNE TOLERANCE ISOANTIGENS LYMPH NODES LYMPHOCYTE CULTURE TEST, MIXED PHYTOHEMAGGLUTININS POKEWEED MITOGENS PREGNANCY PREGNANCY, ANIMAL RATS RATS, INBRED STRAINS SPLEEN T-LYMPHOCYTES TIME FACTORS