Avtor/Urednik		Breskvar, K; Dolžan, V; Lenasi, H; Rudolf, Z; Hudnik-Plevnik, T
Naslov		Analiza polimorfizma citokroma P-450 (CYPIID6), ki metabolizira ksenobiotike pri človeku
Prevedeni naslov		Analysis of polymorphism of human cytochromme P-450 (CYPIID6) involved in xenobiotic metabolism
Tip		članek
Vir		Zdrav Vestn
Vol. in št.		Letnik 63, št. 4
Leto izdaje		1994
Obseg		str. 213-5
Jezik		slo
Abstrakt		Background. The purpose of this work was to introduce a method for categorization of Slovene individuals at risk according to their ability to metabolize drugs and chemical carcinogens; this ability is highly correlated to susceptibility of individuals for chemical carcinogenesis in certain tissues. Methods. The polymorphism of CYPIID6 gene which is one of the genes responsible for metabolism of xenobiotics was studied by a genotyping technique, based on amplification of the corresponding part of genomic DNA by polymerase chain reaction (PCR). DNA was isolated from the blood of 20 blood donors obtained from Blood Transfusion Centre of Slovenia. Results. In the analysed group of samples all possible variants of CYPIID6 gene (homozygous with/without mutations and heterozygous) coding for the enzyme which metabolizes several drugs and chemical carcinogens were detected. 16 out of 20 individuals were found as extensive and 4 as poor metabolizers of drugs. Conclusion. The newly introduced technique is suitable for the use in the medical studies aimed for the prevention of certain cancer diseases.
Deskriptorji		CYTOCHROME P-450 POLYMORPHISM (GENETICS) XENOBIOTICS POLYMERASE CHAIN REACTION BLOOD DONORS GENOTYPE